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KMID : 0351619740150010175
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Kyungpook Medical Journal
1974 Volume.15 No. 1 p.175 ~ p.187
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Effects of Ether Anesthesia on Ultrastructure of the Rat Lung
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Abstract
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This study was undertaken to investigate the ultrastructurai changes of the ling wish special reference to the alveolar lining cells of a rat with single and repeated ether anesthesia.
Twenty healthy albino rats, weighing about 200gm average, were divided into 3 groups.
Group 1. Control group. 2 rats: Untreated.
Group 2. Singh ether anesthesia group. 9 rats: Subjected to moderate anesthesia n-ith ether for 30 minutes.
Group 3. Repeated ether anesthesia group. 9 rats: Subjected to moderate. anesthesia with ether for 15 minutes per day for 3 consecutive days.
Experimental rats were sacrificed at 0 hour (3 rats), ~1 hours(3 rats) and 12 hours (3 rats) after ether anesthesia from the group 2 and 3. Lungs were extirpated and examined using both light and electron microscopes.
The result are as follows:
1) Light microscopic observation:
Lungs showed mild congestion, interstitial edema, thickening of the alveolar septa, and increase of alveolar cells. These changes were more marked in the repeated anesthesia group than in the single anesthesia group.
2) Electron micrescopic observation:
Socn after single ether anesthesia, the type II alveolar. cells were crowded with variably sized osmiophilic inclusion belies which were mostly vacuolated. Some of these inclusion bodies were extruded in to the alveolar lu:en. After 4 hours, these changes were markedly diminished. After 12 hours, the osmiophilic inclusion bodies in the type II alveolar cell showed almost normal pattern except slight increase of their density.
Up to 4 hours after repeated ether bnesthesia, variably sized osmiophilic inclusion bodies were noted in the type II alv~~olr cells. They were considerably increased in number. The density of the osmiophilic inclusicn bodies was markedly increased. After 12 hours, type II alveolar cells showed almost normal appearance e~:cept slight increase of the density in the osmiophilic inclusion bodies.
The type I alveolar cells and the capillary endothelial cells showed no remarkable changes in both experimental groups.
3) These results suggest that ether anesthesia induces severe changes of the osmiophilic inclusion body of type II alveolar cells, but these changes are reversiblc~ and restored to normal within 12 hours.
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